Nuclear protein purification
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- bravebeaker
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- Location: Kobe, Japan
Nuclear protein purification
Hi all,
During nuclear protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add?
Honestly I tried to tackle the problem in different ways but I couldnt, any help would be really appreciated.
Thanks all.
During nuclear protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add?
Honestly I tried to tackle the problem in different ways but I couldnt, any help would be really appreciated.
Thanks all.
AHH, Gravity! Such a cold cruel mistress.
- bravebeaker
- Garter
- Posts: 44
- Joined: Sun May 08, 2011 3:26 am
- Location: Kobe, Japan
Thank you for the elaboration, I know its been almost a month for me to reply I am sorry for that but I finally tried the experiment and the volume I am adding to is 5 ml. But I am not sure how we reached the 2/3 ratio of the original volume.
because 2/3 = .66 and if we multiply (.66)(5ml) I will get 3.3ml to add which is correct but still I dont understand why.
because 2/3 = .66 and if we multiply (.66)(5ml) I will get 3.3ml to add which is correct but still I dont understand why.
AHH, Gravity! Such a cold cruel mistress.
cV = cV right? So, you'll have:
2M . V1 = 5M . V2
2M/5M = V2/V1 -> ratio of original (5M stock) and final (your solution) volumes is 2 : 5. In other words, the 5M solution will make 2 parts of of 5 parts of 2M solution. Thus the solution to which you're adding the NaCl will make 3 parts of it, thus 2/3.
2M . V1 = 5M . V2
2M/5M = V2/V1 -> ratio of original (5M stock) and final (your solution) volumes is 2 : 5. In other words, the 5M solution will make 2 parts of of 5 parts of 2M solution. Thus the solution to which you're adding the NaCl will make 3 parts of it, thus 2/3.
http://www.biolib.cz/en/main/
Cis or trans? That's what matters.
Cis or trans? That's what matters.
- bravebeaker
- Garter
- Posts: 44
- Joined: Sun May 08, 2011 3:26 am
- Location: Kobe, Japan
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