Microparticles and flow cytometry

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Jemi
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Microparticles and flow cytometry

Post by Jemi » Tue Feb 21, 2012 3:30 pm

Hi,

I've been given several flow cytometry profiles to analyse for a piece of work and I'm slightly confused about one of them.

Image

So far I've worked out that it shows microparticles (I think!) and I know that more side scatter means a more complex cell and that Annexin binds to phosphatidylserine but I'm not sure how to interpret it any further than that. I've been told it comes from someone with a disease (which I have to work out) so I thought maybe I could compare it to the same FC profile for a healthy person but I haven't been able to find one.

Any pointers would be hugely appreciated! Thanks!

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canalon
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Post by canalon » Sun Feb 26, 2012 10:30 pm

[Shamed mod note] Arggghh! due to a bad manipulation I erased an answer to this post for no reason. May the author forgive a tired mod and please repost his/her answer. Sorry. Sorry. Sorry. Sorry! [/Shamed mod note]
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

simina
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Post by simina » Thu Feb 28, 2013 5:43 pm

I have a question regarding flow cytometry too!!! Please heeelp. I have a lot of questions based on the cytometry pictures, where they ask to calculate percentage of dead cells, or of CD45R etc. Does anyone know how to guide me through the correct answer???

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JackBean
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Post by JackBean » Fri Mar 01, 2013 5:42 pm

You should probably know how they distinguish them. Don't they tell?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

simina
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Post by simina » Sun Mar 03, 2013 10:54 am

I know in which part of the graph the cells are but I am not sure how to calculate the percentage because I understood that we have to subtract the autoflourescence ones from other graph.. something like that. It is very confusing!!

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Post by simina » Sat Mar 09, 2013 7:53 pm

Why could be the reason of finding more B cells in the splean in comparison with T cells???

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