## CFU conversion

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Holmes
Garter
Posts: 9
Joined: Thu Sep 27, 2012 5:50 am

### CFU conversion

How do I convert mol/CFU (colony forming unit) to millimol?

I came across a research paper saying:
The absolute amount of ATP per bacterium was calculated by dividing mol ATP ml-1 by c.f.u. ml-1.
There was a drop in ATP level (from 3.39±0.216 to 0.59±0.106 mol c.f.u.-1)

I want the ATP concentration (in bacterium) in millimol.
Will it be just 3.39+-0.216 x 10^3 millimol?
Or do I have to do some conversion for c.f.u?

How to do it?

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
you have to keep it in mmol/CFU
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Holmes
Garter
Posts: 9
Joined: Thu Sep 27, 2012 5:50 am
The problem is, I want to use the concentration value somewhere else and I want it to be specified in millimoles.
Is it okay if I just ignore the cfu and use the value as millimol? Will it remain same? I just want the concentration of ATP inside the bacterial cell.

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
Then it's technically per CFU, because each cell theoretically give rise to single colony.
On the other hand, the concentration inside cell will be different from that in media.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Holmes
Garter
Posts: 9
Joined: Thu Sep 27, 2012 5:50 am
Then I think I should just ignore the cfu and convert Mol to mMol, because it clearly specifies that the concentration is 3.39 mol/cfu.
Thanks a lot.

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