Making BL21 competent cells

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
biology_06er
Coral
Coral
Posts: 260
Joined: Tue Mar 21, 2006 9:33 am

Making BL21 competent cells

Post by biology_06er » Sun Feb 12, 2012 9:34 am

Hi there,

I'm making competent cells for the first time and I have to use 20mM Mg2SO4 and 10mM KCl so I'm just wondering how to make these solutions. I assume I make 1M solution of each and to do so I would do the following:

To make 1M Mg2SO4 I would dissolve 144.673g of Mg2SO4 in 1000mL of water but seeing as thats too much if I wanted to 100mL I would dissolve 14.5g in 100mL of water?..Is this correct?

To make 1M of KCl I would dissolve 74.55g in 1000mLs of water but once again 1000mLs is too much so if I wanted to make 100mL i would dissolve 7.4g in 100mLs of water...is this correct?

Cheers,
b_06er

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Feb 14, 2012 3:05 am

Yes
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests