Can filter TBE for reuse?

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genetherapy
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Can filter TBE for reuse?

Post by genetherapy » Sun Dec 25, 2011 8:34 pm

Hi all,
Can I reuse TBE filtering with bottle top filter?
How many times do you reuse TBE for your researchs?

Thanks

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canalon
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Post by canalon » Sun Dec 25, 2011 11:34 pm

For running gels?
No need to waste money filtering. In fact you could have fish swimming in the stuff that would likely not interfere with your gel (but running your gel might make the fish unhappy though). What is important is the buffering ability of the stuff. If your run it at reasonable settings (depends on your setup) TBE can be resused 5 to 10 times (you can see the quality degrading over time), TAE less often (I have often read 5 times max, but since I mostly used TBE anyway...). The main problems you can have are evaporation if you leave it in the cuve.

In general I would reuse without paying attention if I want quick check of my products and make fresh buffer for picture quality gels.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

genetherapy
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Post by genetherapy » Mon Dec 26, 2011 5:18 pm

thanks

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