Can I freeze RNA that is atteched to a column?

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saracanada
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Can I freeze RNA that is atteched to a column?

Post by saracanada » Thu Oct 13, 2011 11:49 pm

Hi everyone,

I have a strange question. I was extracting RNA using Qiamp minikit and at the last step I found out that there was no elution buffer left. So as I had a number of samples, I decided to freeze the columns to which RNA was bound.
Would that be possible to thaw the columns the next day and elute RNA?

Thanks,

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biohazard
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Post by biohazard » Fri Oct 14, 2011 6:49 am

Hehe, the mental image of that situation made me smile :P

Well, RNA is pretty unstable and I am quite certain the recovery is far from optimal after a freeze-thaw cycle, but I guess nothing prevents you from trying (remember to tell us what was the outcome!)

If it was DNA, I think you might be able to do that without much problems.

One option, though somewhat bizarre, would be to fill your columns with some RNA preservation solution that helps to keep RNA strands intact during freezing and thawing before freezing the columns - in case you had some in your lab when running out of elution buffer. I think Qiagen is one of the companies that produces those!

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JackBean
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Post by JackBean » Mon Oct 17, 2011 3:00 pm

by column you probably mean the spin-filters, right? So they cannot be stored in liquid, right?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

saracanada
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Re: Can I freeze RNA that is atteched to a column?

Post by saracanada » Tue Oct 18, 2011 2:33 am

Yes spin-filters, and I assume they can be stored in liquid? not sure...

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Post by saracanada » Tue Nov 15, 2011 9:47 pm

Just to let you know, the recovery was slightly lower but amazing that it worked!

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biohazard
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Post by biohazard » Wed Nov 16, 2011 7:47 am

Cool, thanks for letting us know :)

Btw, did you freeze them with some cryopreservant / RNAse inhibitor, or just as they were?

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JackBean
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Post by JackBean » Wed Nov 16, 2011 4:46 pm

so you left some liquid (buffer?) on top of them?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

saracanada
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Post by saracanada » Mon Nov 21, 2011 4:42 am

No problem!
I didn't leave anything on them! had just frozen the columns! ( too in rush to put solution on)

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