Help! Convert mg/ml to mg/kg

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sadieg
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Help! Convert mg/ml to mg/kg

Post by sadieg » Wed Oct 27, 2010 5:48 pm

Okay, I have nitroglycerin at 5mg/ml (in liquid form).
My mice need a dose of 10mg/kg.
I have 8 mice weighing approximately 30g.
How do I figure out how to dilute to the proper concentration and volume?
I think I need to use C1V1=C2V2, but I don't know how to get the end result.
PLEASE HELP!!!

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canalon
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Post by canalon » Wed Oct 27, 2010 5:52 pm

You do not need C1V1=C2V2.
Your mice need 10 mg/kg and weight 0.03 kg, calculate X (in mg) the total amount to inject.
You have a 5 mg/ml solution divide x by 5 and you know Y the number of ml you need to inject.
Patrick

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any proof. (Ashley Montague)

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Post by sadieg » Thu Oct 28, 2010 12:14 am

I am still confused....I can use c1v1=c2v2...but I guess what I really need to know is how I get C2?
With a C2 of 1mg/ml, and If I am injecting .3ml for a .03kg mouse, then how did I get to this point?
So....
For 8 mice weighing approx 30g each (+a little extra just in case) I was given this information.
C1= 5mg/ml
V1= .8ml
C2= 1mg/ml
V2=4
I need to add saline to the nitroglycerin to get the proper concentration, dose and volume. I'm lost...

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canalon
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Post by canalon » Thu Oct 28, 2010 4:11 am

OK the complete solution for a 30g mouse you need
0.03kg x 10 mg/kg = 0.3mg per mouse
so with your initial solution you are suppose to inject 0.06ml or 60µl.
If you prepare a 1mg/ml solution, 300µl are necessary
Patrick

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any proof. (Ashley Montague)

sadieg
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Post by sadieg » Thu Oct 28, 2010 4:34 am

Ok now that totally makes sense! Thank you so much for your help, I know i must seem absolutely retarded. I swear I am not!

One last question. How would I prepare the 1mg/ml solution with the original concentration that i have which is 5mg/ml nitrolgycerin?

and I also understand how you got to .3mg per mouse, but how did you convert that to 300microliters per mouse? Because that makes sense, we inject .3ml for a 30g mouse.

Thanks you again, i dont know why i cant wrap my head around this

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canalon
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Post by canalon » Thu Oct 28, 2010 1:12 pm

1/ dilute 5 times, or in this case use the c1v1=c2v2
2/ 1mg/ml means that you have 1mg of product in 1ml of solution. if you want 0.3 mg of product, how much solution?

PS: brain usage is not optional.
Patrick

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any proof. (Ashley Montague)

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