Horseradish Peroxidase protein does not SHow up on PAGE gel

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
AlaRoshdieh
Garter
Garter
Posts: 1
Joined: Thu May 20, 2010 2:46 am

Horseradish Peroxidase protein does not SHow up on PAGE gel

Post by AlaRoshdieh » Thu May 20, 2010 2:50 am

Okay, i ran HRP with polyacrylamide gel electrophoresis, the buffer that i used to store the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium dodecyl sulphate (SDS), all my proteins ran succesfuly on the gel except HRP which was NON-EXISTENT after i destained all the coomasie blue. Are there any reasons to why this happened?

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Sat Jun 12, 2010 5:56 pm

I don't think that there is any reason, why shouldn't be one protein visible if is it present in sufficient amount...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Sun Jun 13, 2010 2:53 am

How did you test for the presence of HRP and of the other proteins?
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests