Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
3 posts • Page 1 of 1
Okay, i ran HRP with polyacrylamide gel electrophoresis, the buffer that i used to store the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium dodecyl sulphate (SDS), all my proteins ran succesfuly on the gel except HRP which was NON-EXISTENT after i destained all the coomasie blue. Are there any reasons to why this happened?
Who is online
Users browsing this forum: No registered users and 9 guests