RNA and amine groups ?

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strasbarge
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RNA and amine groups ?

Post by strasbarge » Tue Oct 06, 2009 9:17 am

Hi !

I would like to know if it is possible to link an amine group to a RNA. This is in order to link a fluorochrome and follow its shifting. Or does anyone have any idea if there is a kit to bind fluorochrome to RNA ?

Thanks per adavance for your help !!

Strasbarge

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JackBean
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Post by JackBean » Tue Oct 06, 2009 2:05 pm

Don't know, what kind of fluorochrome, but it definitelly should be possiblle if there are DNA chips and similar stuff ;)
Just join it to either end of the strain. If you wished to use amines anyway, you could join it to the carbonyl groups of basis, so than you will have affected binding to complementary strand ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

strasbarge
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Post by strasbarge » Tue Oct 06, 2009 2:31 pm

Thanks but actually I wanted to know how I could join an amine group to RNA basis precisely... I may not have been clear ^^

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