How does high salt conc. influence denaturation kinetics

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HelloKitty
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How does high salt conc. influence denaturation kinetics

Post by HelloKitty » Tue Sep 22, 2009 2:51 am

How does high salt concentration influence denaturation kinetics of DNA? Explain your reasoning.

Darwin420
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Post by Darwin420 » Thu Sep 24, 2009 4:04 am

Salts can change the charge of the phosphate backbone by donating electrons or possibly attracting an Oxygen atom from the phosphate ion, causing the charge to chage.. which could cause the 5' and 3' end to repel eachother.

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JackBean
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Post by JackBean » Thu Sep 24, 2009 12:23 pm

Doesn't he mean by denaturation just unfolding the strains one from each other?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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Post by Darwin420 » Thu Sep 24, 2009 3:35 pm

yes...and I described that. the 3' and 5' repel each other, thus they unfold.

Look it up in a text.

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Post by Darwin420 » Thu Sep 24, 2009 3:47 pm

But wait, using LOW SALT concentrations is what scientist use to denature DNA. If you have high salt concentrations you will most likely have an unstable z form DNA.

sorry, I am just learning this stuff as well...little rusty.

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