Big dye terminator

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roniadam
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Big dye terminator

Post by roniadam » Thu May 28, 2009 10:32 am

Hi :
I need to sequence a DNA. My primer conc. is 10 micromol and I need to convert it to 3.2 picomol to be able to set uo my sequencing reaction? how can I dilute my primer (10 micromol) to reach this small value of conc (3.2 picomol) ???? Anyone can explain to me in detail please?

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jonmoulton
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Re: Big dye terminator

Post by jonmoulton » Thu May 28, 2009 5:10 pm

(starting concentration) * (starting volume) = (ending concentration) * (ending volume)

or restated more concisely,

C1V1=C2V2

You know your desired ending concentration and volume. You need to find out how much of your starting material to use. So,

V1=C2V2/C1

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