DNA Spin Columns

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 2
Joined: Mon Feb 16, 2009 2:07 pm

DNA Spin Columns

Post by DIWalker » Tue Feb 17, 2009 12:29 pm

I am currently assessing cell lysis techniques that would be most suitable for my research. To do this I want to test lysis separately from purification. Because of this I need a standard DNA purification method which I can use when I change the lysis method I am testing.

I would like to use a DNA spin column as my purification standard. However so far I have had no luck finding any companies that sell the DNA spin columns separate from the lysis buffers in their kits.

Does anyone know where I can buy spin kits seperately? The only ones I have found so far have either been for RNA, to clean up PCR products or to extract DNA from agarose.

User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Feb 17, 2009 3:48 pm

Some companies are selling their kits in pieces. I know that MoBio does. And I remember reading a protocol on how to make your own columns with silica powder (available at your friendly neighbourhood chemical company, I think they were using Sigma) but I do not remember where. maybe it is worth looking for it?

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 11 guests