Discussion of all aspects of cellular structure, physiology and communication.
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Recently, I used FACS-buffer (PBS, 2 mM EDTA, 25 mM HEPES) for antibody dilution and washing. Although, clump formation was less but it was present. Besides, I would also like to know that do the presence of HEPES buffer and EDTA interfere with the result while making antibody dilution buffer.
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