Cell washing

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 1
Joined: Wed Dec 05, 2007 9:24 am

Cell washing

Post by SPatula » Wed Dec 05, 2007 9:34 am

I have a question

I know you can wash cells by using PBS or a Sodiumchloride solution (I know there are probably more, but these are the ones we've got here).
Some people here use PBS others the Sodiumchloride solution, but are there exceptions when you can use one but not the other?

User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Wed Dec 05, 2007 4:45 pm

PBS: phosphate buffered saline.
Sodium chloride solution: saline (unbuffered).

One is easier to make, but can be a problem if there is some sort of pH sensitivity. Although I guess that if your assay is phosphate sensitive, PBS might not be a good idea.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

User avatar
King Cobra
King Cobra
Posts: 885
Joined: Fri Jun 15, 2007 7:03 pm
Location: San Diego, Ca

Post by MichaelXY » Wed Dec 05, 2007 9:30 pm

I think PBS would precipitate if calcium is present, not sure about Iron.

Posts: 493
Joined: Fri May 27, 2005 8:58 am
Location: Pilipinas a.k.a. Three Stars and a Sun (300, 000 sq Km)

Post by mcar » Thu Dec 06, 2007 1:58 pm

It depends. if there are chemicals that could dehydrate cells quickly or could disintegrate cell contents--gotta go to a next technique that is suited in studying cells. Normal saline solution afterall is isotonic.
---Just one act of random kindness at a time and you can change the world---

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests