What Influences transcription rate in bacteria?

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What Influences transcription rate in bacteria?

Post by neon » Mon Jul 10, 2006 3:19 pm

Hello (for the second time today :wink: )

I am keen to determine the factors influencing the rate of transcription in microbes (especially e.coli). To be more precise, I am looking to discover factors affecting protein production, so any factors influencing transcription, translation and PTM etc. are important.

Something I am particularly keen to ascertain, is whether particular chemical concentrations are influential during transcription, translation etc. Does the concentration of (recently) synethesised proteins influence transcription?
I believe that excessive levels of phosphor within the nucleus cause the open complex to close - so presumably phosphor concentration is one of the factors I wish to determine.
Am I correct here, or way off target?

The reason I ask this, is that I am building a [abstract] model of bacteria, for which the details of protein producation are of paramount importance. I assume there is an influence of many things on the rate of transcription (nuclease concentration etc.). The model is based on the operon, thus genetic regulation is inherent from the interaction of transcription factors but I am wondering what other factors to include.

Thanks in advance for your suggestions.


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Post by seaseasea » Wed Jul 12, 2006 7:32 am

Hello neon,
This is the second time today I meet you.
I still want to say lots of factors influencing transcription rate in bacteria.
You could look up monograph or other references.
Hope this helps!
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Happiness is also God!

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Post by ucsf_student » Thu Jul 13, 2006 8:40 pm

Hi Neon,

For starters bacteria do not have nuclei!

Proteins such as transcription factors that activate can either help RNA polymerase bind to the promoter or help open complex formation. (alternate sigma factors are also something you should think about-- small molecules such as ppGpp can afect the affinity of the polymerase for certain sigma factors) also mRNA can degrade of course. protein production depends on the strength of the ribosome binding site. proteins can also degrade.

A good review:
The regulation of bacterial transcription initiation
DF Browning, SJW Busby
Nature Reviews Microbiology, 2004

Good luck!

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