Porin-deficient mutant

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Layd33foxx
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Porin-deficient mutant

Post by Layd33foxx » Fri Apr 06, 2012 5:26 am

:?: Some bacteria become resistant to tetracycline because they don't make porins. Why can a porin-deficient mutant be detected by its inability to grow on a medium containing a single carbon source such as succinic acid?

AstraSequi
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Post by AstraSequi » Sat Apr 07, 2012 6:44 am

What is the function of a porin?

Knowing that, what sort of experiment might you design to see whether or not the porin is present? A system where the cell dies if the porin is not present (that is, the cell can't execute the porin's function) is usually very convenient.

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Re: Porin-deficient mutant

Post by Layd33foxx » Sun Apr 08, 2012 5:33 pm

Porins are channels control the transport of substances in and out of the cell, imparting the membrane its property of semipermeabililty.

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Re: Porin-deficient mutant

Post by Layd33foxx » Sun Apr 08, 2012 5:45 pm

Bacteria can develop resistance to the antibiotic by mutating the gene that encodes the porin – the antibiotic is then excluded from passing through the outer membrane

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Post by AstraSequi » Wed Apr 11, 2012 2:37 am

Now suppose that the porin actually transports more than one substance - say, it can act on succinic acid as well as tetracycline. How might you figure out whether the porin is working or not? :)

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