Identifying unknown bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Identifying unknown bacteria

Post by alison_023 » Sun Nov 04, 2007 12:50 am

I am trying to figure out what my unknown bacteria is for microbiology. I think I have narrowed my choices down to 3: Bacillus megaterium, Bacillus cereus, Bacillus stearothermophilus. I need help choosing which one because some of my test dont match up.

Colony description:
Size- small and some pinpoint size
Shape- round
Color- white, non translucent
Consistancy- Glossy shinny

Cell morphology/ arrangement- bacillus/ individual, short chains
Staining Reactions- gram positive
Motility- yes
Endospores- yes
oxygen requirements- aerobic

Extracellular enzymes:
Starch- negative
Gelatin- positive
Casein- positive
Tributyrin- positve

Glucose- acid only
Sucrose- acid only
Lactose- acid only

Hydrogen sulfide- negative

IMViC Test:
indole- negative
methyl red- negative
voges-proskauer- negative
citrate- negative

Catalase- positive

Can anyone help me out on which one to choose? Or do you know of any other test I could run?

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Post by mith » Sun Nov 04, 2007 1:11 am

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