Acid and gas from lactose

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Acid and gas from lactose

Post by jessi73 » Fri Oct 26, 2007 4:23 am

Hi everyone!
I am trying to find the appropriate test that will tell me whether my organism makes acid and gas from lactose or not. I looked all over the place and found that a lactose fermentation test would determine that.... just checking in to see if I'm on the right path??
Thanks :-)

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King Cobra
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Post by victor » Fri Oct 26, 2007 9:47 am

just use the lactose broth in the test tube which is also filled with Durham tube. Don't forget to put a methyl red indicator in it.
After incubation for 2-3 days in 37C, if the broth turns yellow, then your bacteria fement lactose.
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