biochemistry-enzyme kinetics lab help

Debate and discussion of any biological questions not pertaining to a particular topic.
New forum link: https://forum.biologyonline.com/index/c ... discussion

Moderators: honeev, Leonid, amiradm, BioTeam

The new Biology Online has arrived!

We are very pleased to announce the website's new design and features. As a biology website pioneer in 2001, it has grown to become the world's most comprehensive online database for biology terms and topics. Thus, we would like to invite you to take part in this significant milestone by checking out these latest features from Biology Online.

Biology Dictionary

The largest online biology dictionary with over 70,000 biology terms! Learn various topics, concepts, and principles in biology. Start by searching here.

Biology Articles

Get the latest biology news, insights, trends and discoveries in biology and other related research fields. We have over 5,000 articles you can choose from.

Biology Tutorials

From the basics to the more advanced lessons, our tutorials will help you learn various topics in biology. Browse from over 100 free biology lessons here.

New Biology Forum

We hold one of the largest discussion boards in biology online! Join our community by signing up here or log in here if you already registered with us before.

Thank you and we hope you will join us as the site continues to grow by leaps and bounds.

Sincerely,
Biology Online Team

Locked
nickname
Garter
Garter
Posts: 17
Joined: Thu Nov 19, 2009 3:10 am

biochemistry-enzyme kinetics lab help

Post by nickname » Thu Nov 19, 2009 3:16 am

Hello,

I had to do a lab on enzyme kinetics: finding Vmax and Km for alkaline phosphatase.

What are some sources of error that could be found when conducting this kind of experiment?

I know that temperature could be one of the errors, but could you please help me find other types of errors encountered in enzyme kinetics experiments?

Thanks for your help!

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Thu Nov 19, 2009 4:10 am

E.g. pH. Like if you set pH of your buffer to the optimal pH, but it's not strength enough and some of the components added to reaction will change it. Also, the reaction itself can change pH (that's why you use buffers;) and especially phosphatase reaction will change it) and if it changes pH in the direction, where is your buffer weakened, than you are in trouble ;)
Of course, bad pipeting, e.g. if you dilute your substrate wrong, you will get bad results ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

nickname
Garter
Garter
Posts: 17
Joined: Thu Nov 19, 2009 3:10 am

Post by nickname » Wed Nov 10, 2010 10:51 pm

Thank you so much for your help!

Locked