DGGE - DNA migration

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 2
Joined: Sat Apr 30, 2005 1:36 pm

DGGE - DNA migration

Post by TomMol » Sat Apr 30, 2005 1:44 pm


Two months ago I started use DGGE method. I want to use it to detection bacteria (mainly Lactobacillus) in stool. I have got problem. When I put PCR product into wells samples didn’t migrate equally. It migrate faster on both periphery gel and slower in center. I added 100 ul 10% APS and 10 ul TEMED per 25 ml poliacrylamide. I used 100 V and temperature 60C – 10 % gel.

What should I change in my procedure to my results will be better?
Thank you


[email protected]

User avatar
Posts: 16
Joined: Fri May 20, 2005 3:02 pm
Location: Europe

Post by Niqqie » Fri May 20, 2005 6:18 pm

Either it is a problem in the way the electricity is flowing through your gel, or it is a problem of how the gel polymerizes. I personally think 100V is a lot, I run 7% polyacrylamide gels at 60V; I only use a high voltage to get the samples into the gel, after which I turn it down.
more sciences at physicsforums.com

Post Reply

Who is online

Users browsing this forum: No registered users and 8 guests