southern blot question

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kabuto
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southern blot question

Post by kabuto » Wed Aug 23, 2006 12:18 pm

what is the importance of the following solution
a) depurination
b) neutralisation
c) denaturation

i think for a and c, it is to unwind the the double stranded dna

pls tell me if i'm right or not
and why does it has to be neutralised

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kabuto
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Post by kabuto » Wed Aug 23, 2006 12:26 pm

and also the function of 10X SSC :?

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