Checking overexpression of genes in tumor cell lines

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curious1
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Checking overexpression of genes in tumor cell lines

Post by curious1 » Fri Jun 16, 2006 1:39 am

Hi,

This might seem like a trivial question but I was wondering is a cell line automatically derived from a tumor? If a cell line is by definition, immortal how can there be a normal cell line? If I wanted to check whether particular genes were overexpressed in tumor cell lines how would I go about doing this? Do I compare the expression level of these genes with normal cell lines or just designate a calibrator within the tumor cell lines (like tumor cell line giving me the lowest expression level) and just compare the rest of the tumor cell lines to this? How do I know if it is overexpressed? :?: :?: :?:

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