Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Post by summer » Wed Mar 16, 2005 3:38 pm

links and info of RT- PCR?

appreciate for any inputs.

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Post by DevGrp » Thu Mar 17, 2005 9:43 am

just got an email from Promega advertising their ALL NEW applications guide, haven't checked it but I always found the old paper edition very useful witha good section on RT-PCR


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Post by Chris4 » Thu Mar 17, 2005 9:48 am

Reverse transcription polymerase chain reaction. PCR with reverse transcription
Reverse transcriptase-PCR (RT-PCR) is a powerful tool for gene expression analysis at the RNA level, primarily due to its sensitivity, speed, ease of use, and versatility. In a typical RT-PCR procedure, a reverse transcriptase is used to generate a complementary DNA (cDNA) copy of RNA molecules, followed by polymerase chain reaction (PCR) to amplify the cDNA. Since the PCR process part is usually robust and generic, performance of a given RT-PCR system is mainly determined by the activity of the reverse transcriptase used.
Copied from here: http://www.sigmaaldrich.com/Area_of_Int ... T_PCR.html
Heres a nice animation of how to perform RT-PCR http://www.bio.davidson.edu/courses/gen ... T_PCR.html
info on RT-PCR optimization http://www.promega.com/guides/pcr_guide ... omega.html
(May be too advanced)
Hope that helps. Is should also be in a genetics or molecular biology text book.

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