Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- King Cobra
- Posts: 3501
- Joined: Thu Jul 07, 2005 7:58 am
- Location: 55284 Yogyakarta, Indonesia
dapi? You mean DAP, the chromogen substance? In my protocol, I add DAP after applying secondary antibody because it is a marker to see whether there is bond between antigen and primary antibody (monoclonal), which can be detect by bond between that primary antibody and secondary antibody (polyclonal). Thus I think, the secondary antibody must be placed before the chromogen. The principle of IHC or ICC is similar to ELISA's.
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