cAMP Forskolin

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ms123
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cAMP Forskolin

Post by ms123 » Thu Feb 25, 2010 8:58 pm

I'm treating TF-1 cells with forskolin and measuring cAMP increase by ELISA, but I have not been able to see a significant increase with varying concentrations of forskolin. I've tried multiple treatment volumes and concentrations, and I'm using cell count dictated by the ELISA. 1x10^7 cells/mL. Has anyone else had this trouble in TF-1s or does anyone have any helpful tips? Thanks.

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