Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Hello everybody. I was just wondering if there any formula to calculate the necessary amount of DNA for effective transformation. Maybe someone know where to look in to get this information. Thank you
not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.
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