Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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You should be OK overnight in the standard destain of 30% MeOH, 10% HOAc. It is possible to completely destain a coomassie-stained gel, but you usually up the MeOH to 40-50% and go for at least 24 hours. You can leave the gel in stain overnight and destain in the morning, or transfer a partially destained gel to water for the overnight, and continue with destain in the morning. All of that works.
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