Search found 227 matches

by wbla3335
Tue Jun 12, 2012 8:09 pm
Forum: Zoology Discussion
Topic: any clues what this is, please?
Replies: 7
Views: 7393

Re: any clues what this is, please?

My guess is an air bladder.
by wbla3335
Thu May 31, 2012 4:02 am
Forum: Evolution
Topic: 5 best proofs of evolution
Replies: 170
Views: 347686

Re: 5 best proofs of evolution

Jorge, you are free to believe that anyone who does not share your opinions is stupid or ignorant, but it is impolite to say so. A few other adjectives also come to mind.
by wbla3335
Wed May 30, 2012 11:18 pm
Forum: Evolution
Topic: 5 best proofs of evolution
Replies: 170
Views: 347686

Re: 5 best proofs of evolution

Various ravens, Many people adhere to a strict application of the word "proof". I am one, but I can also accept the concept "most likely true". Much of science deals with the latter, and so many professionals with much better qualifications than Jorge describe evolution as a fact...
by wbla3335
Wed May 23, 2012 7:59 pm
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 7985

Re: ligation problem although try everything,suggestions?

Vortex the buffer, not the enzyme.
by wbla3335
Wed May 16, 2012 5:14 pm
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 7985

Re: ligation problem although try everything,suggestions?

I haven't done any lab work for many years, but in the old days, the buffers that came with ligases would often form a precipitate and needed to be vigorously vortexed before using. Have a look at your buffers to see if there's any precipitates. If so, try vortexing the **** out of them before use.
by wbla3335
Tue May 08, 2012 2:10 am
Forum: General Discussion
Topic: The Primal/Paleo Diet? Or the Parasite Diet?
Replies: 3
Views: 6621

Re: The Primal/Paleo Diet? Or the Parasite Diet?

I won't even eat sushi (how many sushi "chefs" are properly trained?). Most parasites are rather species-specific and cannot survive in atypical hosts. But there are plenty of parasites that can live in us - enough to keep me away from uncooked or improperly cooked meat/animals. Carrots ar...
by wbla3335
Sun Apr 29, 2012 7:48 pm
Forum: Human Biology
Topic: The human head and the concentration of sensory devices
Replies: 3
Views: 4249

Re: The human head and the concentration of sensory devices

It's called cephalisation (replace the "s" with a "z" if you're a Yank). An internet search should return plenty to answer your questions.
by wbla3335
Mon Apr 23, 2012 11:29 pm
Forum: Molecular Biology
Topic: 3' end of a PCR primer
Replies: 2
Views: 1778

Re: 3' end of a PCR primer

Too many Gs or Cs can encourage mispriming. The ones that match can bind strongly, thereby increasing the chances that mismatches at other positions are tolerated.
by wbla3335
Mon Apr 16, 2012 10:58 pm
Forum: Genetics
Topic: Isolation of a Gene of Interest for Subcloning
Replies: 2
Views: 2255

Re: Isolation of a Gene of Interest for Subcloning

Design insert-specific primers with restriction sites added to the ends that the second vector has. PCR the insert, digest the products and the second vector, and ligate.
by wbla3335
Sat Apr 14, 2012 11:34 pm
Forum: General Discussion
Topic: Loss of genetic diversity
Replies: 8
Views: 10202

Re: Loss of genetic diversity

nucleotide diversity ϴ = 4N(e)µ, where N(e) is the effective population size and μ is the mutation rate per generation
by wbla3335
Sun Apr 01, 2012 2:47 am
Forum: Evolution
Topic: Oh God, a debate!
Replies: 14
Views: 10290

Re: Oh God, a debate!

No.
by wbla3335
Sun Apr 01, 2012 2:46 am
Forum: Evolution
Topic: What's performance testing?
Replies: 1
Views: 2280

Re: What's performance testing?

http://www.ncbi.nlm.nih.gov/pubmed/8878686 This study always impressed me as a good illustration of how much variation can exist in a population upon which selection can act.