Search found 145 matches

by magicsiew
Sat Dec 18, 2010 8:16 am
Forum: Microbiology
Topic: Virus protein coat
Replies: 2
Views: 2611

Yes, you may do that, try to collect as much sequences as possible from related species of your particular gene and find the conserved region.
by magicsiew
Fri Dec 17, 2010 9:59 pm
Forum: Molecular Biology
Topic: Primers design problem
Replies: 5
Views: 3132

Primers design problem

Hi, another post on primers design. I have to design primers to amplify a gene, but then has no annotation of that particular gene in Genbank. So what I do is find the gene sequence from related species-found 6 protein sequences and have only these 6 available (even less in nucleotide sequences). Wh...
by magicsiew
Fri Dec 17, 2010 5:32 pm
Forum: General Discussion
Topic: Postgraduate degree
Replies: 12
Views: 6954

Re:

Darby wrote:A lot of US grad schools go straight through to the PhD from an incoming Bachelor's.


Hi Darby, you mean they only take 1st class honour Bachelors to go straight throught to the PhD, or they got some other regulation?
by magicsiew
Fri Dec 17, 2010 5:31 pm
Forum: General Discussion
Topic: Postgraduate degree
Replies: 12
Views: 6954

Re:

OK, so you might be interested in the French system then Since I have moved to Canada I might not be fully up to date but what I can tell you should still be true. You should find detailed informations here: m Hi Canalon, I have looked through on the courses I am interested. From the admission site...
by magicsiew
Fri Dec 17, 2010 3:46 pm
Forum: General Discussion
Topic: Postgraduate degree
Replies: 12
Views: 6954

I am from Malaysia, few preferred places will be US, UK, Australia, New Zealand, French and Spain. My undergraduate course is 3 years (honour).
by magicsiew
Fri Dec 17, 2010 1:30 pm
Forum: Botany Discussion
Topic: what is flowers?
Replies: 8
Views: 8185

Re: what is flowers?

gyx2650 wrote:It looks like the flowers of Hylocereus undatus , its fruit is Pitaya.
We call it dragon fruit. :)


Agree, it looks like dragon fruit
by magicsiew
Fri Dec 17, 2010 1:27 pm
Forum: General Discussion
Topic: Postgraduate degree
Replies: 12
Views: 6954

By the way, i found that some University only take undergraduate and PhD candidate. Means for those who wish to take post graduate degree must be first class honour year or take Master degree at other university first right?
by magicsiew
Fri Dec 17, 2010 1:19 pm
Forum: General Discussion
Topic: Postgraduate degree
Replies: 12
Views: 6954

Postgraduate degree

Hi, for postgraduate degree, I would like to ask, is there any other method to jump from undergraduate to PhD, besides getting first class honour year?
by magicsiew
Tue Dec 14, 2010 3:59 pm
Forum: Molecular Biology
Topic: DMSO use in PCR
Replies: 4
Views: 4664

Why do u want to UV treat your master mix? I am not sure will it affect the master mix, since there are dNTP in your master mix. And did you put it on ice during the whole period while UV-treating?
by magicsiew
Tue Dec 14, 2010 10:11 am
Forum: Bioinformatics
Topic: E value confidence
Replies: 4
Views: 5174

I clone out 2 genes with 0.074 and 0.006 e value respectively. They are totally different species, banana against arabidopsis in Genbank database. My sequences are 850bp and 1kb in length. The coverage are 2%, very small only, max score is 42.8 and max identical is 98%. At 1st, I think of doing RACE...
by magicsiew
Mon Dec 13, 2010 7:49 pm
Forum: Bioinformatics
Topic: E value confidence
Replies: 4
Views: 5174

E value confidence

Hi, I have cloned out a gene that without annotation of my particular species in GenBank, when I blast the sequences, able to find the same gene from related species. But the e value are 0.074 and 0.006 respectively. By just analyzing from this data, can I conclude I have successfully clone out the ...
by magicsiew
Sun Dec 12, 2010 1:56 pm
Forum: Ecology
Topic: how is pollination an example of mutualism?
Replies: 8
Views: 23132

When the bees uptake honey from the flowers, the pollen stick on its legs to be transfer to other place.