Search found 145 matches

by magicsiew
Tue Jan 18, 2011 2:28 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Hi JackBean, I have looked through the protocol of the hybridization method. My sample genome size is very huge, if I were to build the library, I doubt how many hundreds, or thousands of plate I will require to make before getting the library done. In this case, it doesn't seem like a good alternat...
by magicsiew
Tue Jan 18, 2011 2:25 pm
Forum: Molecular Biology
Topic: How can I extend my sequencing results?
Replies: 8
Views: 3554

Re:

canalon wrote:Sequence walking: Design a new primer at the end of the one you have.


Hi, if I need to elongate the 5' direction?
by magicsiew
Tue Jan 18, 2011 2:19 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Hi JackBean, I have looked through the protocol of the hybridization method. My sample genome size is very huge, if I were to build the library, I doubt how many hundreds, or thousands of plate I will require to make before getting the library done. In this case, it doesn't seem like a good alternat...
by magicsiew
Mon Jan 17, 2011 4:04 pm
Forum: Molecular Biology
Topic: How can I extend my sequencing results?
Replies: 8
Views: 3554

carried out RACE-PCR
by magicsiew
Mon Jan 17, 2011 9:42 am
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Hybridization...
by magicsiew
Sun Jan 16, 2011 6:18 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

I think I will go for the second method.. Thanks :)
by magicsiew
Sun Jan 16, 2011 4:52 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Hmm... I am not sure if I get your meaning right? Are you saying making DNA library, sequence them, then use the fragment as probe, align them and spot the region for full length of the gene? If this is what you meant, will it be more expensive than RACE-PCR? Say if I only need to carry out 4 RACE-P...
by magicsiew
Sun Jan 16, 2011 3:00 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

But there is no DNA library yet... At least now still dont have...
by magicsiew
Sun Jan 16, 2011 10:10 am
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Able to find partial sequence in the EST only
by magicsiew
Sat Jan 15, 2011 3:59 pm
Forum: Molecular Biology
Topic: Alternative to RACE-PCR
Replies: 18
Views: 7007

Alternative to RACE-PCR

Hi, to clone out full length of a gene from a genome without complete sequencing, the way I know can use is perform RACE-PCR. However, RACE-PCR very expensive. Besides this method, is there any other method to clone out the full length of gene? One method I have thought about is designed another pri...
by magicsiew
Fri Jan 14, 2011 7:33 am
Forum: Genetics
Topic: Punnett Square Question
Replies: 5
Views: 3455

Maybe u can count ur hypothesis in a chi square test, if the hypothesis is accepted, then u can argue in that way.
by magicsiew
Fri Jan 14, 2011 1:40 am
Forum: Genetics
Topic: Punnett Square Question
Replies: 5
Views: 3455

Re:

There isn't any more information, but I'm pretty sure this assignment doesn't cover the variations to Mendel's Principles. Just the basic, "pea plant" scenarios. (I'd thought of lethal genes too.) If you are saying pea plant scenarios, then u are saying Mendelian (Mendel's principles), no...