Search found 5663 matches

by JackBean
Thu Sep 17, 2009 5:00 pm
Forum: General Discussion
Topic: H2O on both sides of eq'n?
Replies: 1
Views: 707

Hi,
that's because the water is in the beginning decomposited to (activated) hydrogens and (molecular) oxygen by light harvesting complex and later on, during the Calvin cycle, it is again produced, but in another, independent reaction ;)
by JackBean
Wed Sep 16, 2009 6:36 pm
Forum: Cell Biology
Topic: Protein half-life
Replies: 3
Views: 4233

You mean very slow degraded proteins? :-P

What information you mean?
by JackBean
Wed Sep 16, 2009 6:35 pm
Forum: Molecular Biology
Topic: The term "degeneracy of genetic code"
Replies: 1
Views: 4845

Hi, well, I haven't think about that in this way anytime earlier But if you look e.g. here: m there is also meaning "declination, decline, decay, deterioration" and its antonyms: development, improvement. This is, in my opinion, meaning of this word in this context. Also, if you remember f...
by JackBean
Wed Sep 16, 2009 6:38 am
Forum: General Discussion
Topic: Name game
Replies: 176
Views: 215969

Let's not end with 's' anymore :)

Sedum rupestre - rozchodník suchomilný; Gewöhnliche Felsen-Fetthenne; in English probably Angelina or Rocky Stonecrop
http://www.biolib.cz/en/taxon/id169418/
by JackBean
Wed Sep 16, 2009 6:16 am
Forum: Microbiology
Topic: Methods Identifying Unknown Bacteria?
Replies: 10
Views: 14961

Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and see if it still can grow on it? Might be, but this takes...
by JackBean
Wed Sep 16, 2009 6:09 am
Forum: Microbiology
Topic: Synthesis of thermostable Archaeal membrane
Replies: 2
Views: 1958

by JackBean
Tue Sep 15, 2009 6:10 am
Forum: Genetics
Topic: Genes Interation
Replies: 2
Views: 2081

Hi, the difference is in whether the novel phenotype is dominant or recesive. The Line 1 is recesive, so when you breed it with leafy plant, it predominats and small plants have leaves. On the other hand, Line 2 is dominant, so even, when you breed it with leafy plant, the new plants will miss leave...
by JackBean
Tue Sep 15, 2009 5:50 am
Forum: Molecular Biology
Topic: DNA sequencing
Replies: 1
Views: 815

by JackBean
Tue Sep 15, 2009 5:48 am
Forum: Cell Biology
Topic: Propagule size and number produced???
Replies: 7
Views: 5323

Basically, you are right. If you can produce lot of babies, than you do not need to take care of them, because some will by some chance survive. On the other hand, If you take care about your child for many, many years (like humans or elephants), than you are not able to take care about much more th...
by JackBean
Mon Sep 14, 2009 8:22 pm
Forum: Bioinformatics
Topic: SDS-PAGE help please
Replies: 6
Views: 17332

Re: SDS-PAGE help please

Hi, first of all, SDS does NOT break proteins into smaller peptides. As you wrote, aminoacids can have negative/positive charge, so if you put it into electric field, it will move to electrode with the other charge. For this reason you add SDS, which binds to all proteins in approx. the same ratio a...
by JackBean
Mon Sep 14, 2009 8:05 pm
Forum: Cell Biology
Topic: Metal chelate chromatography
Replies: 0
Views: 2022

Metal chelate chromatography

Hi all, is there anybody with experience with metal chelate chromatography ( immobilized metal ion affinity chromatography , IMAC ). I wished to try it as one (or more) step(s) in my purification protocol and tried to study in the 2nd edition of Protein purification textbook, but it didn't work very...