Search found 16 matches

by Jumpshooter
Thu Dec 15, 2005 3:48 pm
Forum: Molecular Biology
Topic: Genes and Proteins
Replies: 10
Views: 5585

The Role of Introns

*Yeah, pretty much like all the members have said: Introns are non-coding DNA in the chromosomes. Their main role is as a Structural support to keep the DNA phsyically stabilized--sorta like scaffolding. You use it to support you while cleaning your gutter or painting your home. Introns are structur...
by Jumpshooter
Thu Dec 15, 2005 3:40 pm
Forum: Molecular Biology
Topic: What purpose is served by cutting the onion into
Replies: 4
Views: 4026

cutting that union

*In any biochemical analysis procedure you want to optimise the conditions for the assay. When you cut up your sample into very small peices you are Increasing the Number of Sample Particles which in turn increases the surface area for the chemical reagents to extract the DNA from. You will increase...
by Jumpshooter
Wed Dec 14, 2005 9:51 pm
Forum: Molecular Biology
Topic: Master in Molecular Medicine
Replies: 2
Views: 1863

go for the phd instead

of the MS degree. You will find that the master degree will "over qualify" you for entry level technician jobs; whereas the phd will help you earn more money. But be ye prepared to do a 3 year post doc.
by Jumpshooter
Wed Dec 14, 2005 7:32 pm
Forum: Molecular Biology
Topic: RNA contamination in spec analysis
Replies: 3
Views: 2574

Your DNA extraction was good, Astha

you got a26/280 ratio of greater than 1.5 indicating less protein contamination; however, I'd suggest you also run a picogreen assay to determine exactly how much dsDNA template you have in that sample. The a260-280 is telling you the Total level of nucleotides (rna, ssdna, oligonucleotides, free nu...