Search found 43 matches

by Fornita
Wed May 18, 2016 3:14 am
Forum: Genetics
Topic: CRISPR/Cas technology
Replies: 1
Views: 10235

For stable cell, I used to order service from Biogene. Here is the url, you can contact them from:
http://www.creative-biogene.com/Service ... Lines.html
And if you want use utilize CRISPR technology for animal model, you may contact the company, Animodel. I did not remember the company url, you can search the result on google.
by Fornita
Wed May 18, 2016 3:01 am
Forum: Genetics
Topic: Knockdown vs Knockout
Replies: 3
Views: 9710

Easily speaking, knock-down for short time, transient change, you reduce, maybe, 50% the effect of the gene. Knock-out for inherit change, for longer time, you reduce the effect of the gene to 0%.
by Fornita
Wed May 18, 2016 2:43 am
Forum: Genetics
Topic: Is talent inherited?
Replies: 44
Views: 76418

In my opinion, inherit part and environment are all needed for talents. Inherit part contribute the physical fundamental for talents. And environment determine whether the "talents" could become real talents.
by Fornita
Tue May 17, 2016 3:18 am
Forum: Cell Biology
Topic: CHOICE OF MAMMALIAN CELL HOST
Replies: 1
Views: 6446

Re: CHOICE OF MAMMALIAN CELL HOST

:D :D
Thanks for your share.
Do we need to consider the relationship between the host cell, vector and protein we want to expression? Sometime good choice to lead to high expression of protein, but bad choice will result less protein amount. So how shall we chose them?
by Fornita
Tue May 17, 2016 3:03 am
Forum: Cell Biology
Topic: Do we know all the proteins (and their primary structure!)?
Replies: 7
Views: 12006

Re:

We do not know every protein sequence. The genes do not code to the post traductionnal modifications. As exemple, on the collagen sequence, we have a repeat of proline but in the real molecule, some aa are hydroxyproline. In addition, some protein need to loose a peptide to be active or to modify t...
by Fornita
Tue May 17, 2016 2:49 am
Forum: Cell Biology
Topic: PEG principle
Replies: 2
Views: 4058

You can search the PEG principle directly on google search. However, if you want to do the experiments yourself, you'd better search papers did the relevant experiment. You can find details and steps in papers. I think that will be more useful. For example, a paper on NCBI: http://www.ncbi.nlm.nih.gov/pubmed/16761719
by Fornita
Tue May 17, 2016 2:40 am
Forum: Cell Biology
Topic: Are cells on agar or in agar?
Replies: 7
Views: 7166

Re: Are cells on agar or in agar?

tumorigenic eukaryotic cell form colonies in soft agar. However I do not believe that non tumorigenic (and even tumorigenic) do it in more solid agar. If cells grow on or inside solid agar why are we all using culture medium ? Bacteria grow inside soft agar and on solid agar. Am I wrong ? We use di...