Search found 30 matches

by paperclip
Thu Sep 22, 2005 5:16 am
Forum: Cell Biology
Topic: Sea squirt blood cells
Replies: 0
Views: 1842

Sea squirt blood cells

Vanadium is found in sea squirt blood cells (vanadocytes). Vanadium is V3+ and VO2+ in the blood cell vacuole. The function of vanadium may be as a sink for protons and electrons, not dioxygen carriage (used to be considered haemovanadin, probably still is ), vacuole pH was about 1 due to the presen...
by paperclip
Mon Aug 22, 2005 7:56 am
Forum: Molecular Biology
Topic: DNA-bound AP Endonuclease (APE1) - DNA Repair
Replies: 3
Views: 2966

Perhaps we can take Ka to mean how many times the acid flips between the two states of acid and base per second. Then can we make the reaction take place more readily?
by paperclip
Sun Aug 21, 2005 9:19 am
Forum: Molecular Biology
Topic: DNA-bound AP Endonuclease (APE1) - DNA Repair
Replies: 3
Views: 2966

DNA-bound AP Endonuclease (APE1) - DNA Repair

This is odd.

Asp 210 acting as a Lewis base in the active site causes deprotonation of water creating a hydroxyl ion that acts as a nucleophile attacking the phosphate group resulting in cleavage of the scissile P-O3' bond.

Isn't Aspartate acidic and water relatively basic?
by paperclip
Mon Aug 15, 2005 2:33 pm
Forum: Molecular Biology
Topic: molecular weight
Replies: 11
Views: 5614

Alcohols may undergo a wide range of reactions. The presence of an oxygen atom with its two pairs of electrons means that alcohols may act as nucleophiles. However, amongst the most important reactions are those which lead to the loss of water (dehydration reactions), and those which lead to the for...
by paperclip
Mon Aug 15, 2005 1:53 pm
Forum: Molecular Biology
Topic: molecular weight
Replies: 11
Views: 5614

Suppose we throw in some alcohol and raise the pH?
by paperclip
Mon Aug 15, 2005 12:56 pm
Forum: Molecular Biology
Topic: molecular weight
Replies: 11
Views: 5614

pH adjusting is necessary because it will create a favorable environment which such substance, for instance enzyme, will work properly. For example, Tris-HCl should be adjusted into pH 7.5 to get the optimal DNase and RNase activity in your assays. OK. What happens if we're to alter the PH. Does it ...