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Dear Biohazard, First of all I got 2 separate populations (2 peaks) in lymphocyte population when stained thawed PBMCs without incubation of cells (I used 5 mM stock solution for 1 million cells and incubate for 10 min according protocol C34554, Molecular Probes from Invitrogen). I expected not to h...
- Tue Nov 08, 2011 10:29 am
- Forum: Cell Biology
- Topic: CFSE proliferation assay
- Replies: 3
- Views: 5980
Hi everybody! I am staining thawed PBMCs with 5uM stock solution of CFSE for proliferation assay and also for control of the staining. However, in the results from Flow Cytometry of day 0 samples, (i.e. freshly stained and not cultured) there are 2 separated picks in lymphocyte population. Does anyo...