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by johnmay
Mon Oct 17, 2011 4:43 pm
Forum: Molecular Biology
Topic: question about LCM
Replies: 0
Views: 2786

question about LCM

Hello all, Recently I am set to perform LCM on PFA-fixed midbrain sections of TH-GPF transgenic mice and encountered some problems. The quantity and quality of RNA isolated from LCM cells were too poor to do the downstream analysis like real-time PCR. Because the GFP expression in the cells of inter...