Search found 4 matches

by kenpier
Tue Sep 27, 2011 5:33 am
Forum: Microbiology
Topic: Making Competent Cell
Replies: 5
Views: 5404

When preparation of competent cell, which freezing well be better?
Dry Ice or Liquid N2?
by kenpier
Thu Sep 08, 2011 3:18 am
Forum: Microbiology
Topic: Making Competent Cell
Replies: 5
Views: 5404

The competency level of the cell don't always hit 1x10exp8 to 1x10exp9 cfu's/ug.

Sometimes is just too low, result are not always consistent. Is there anything to take note?
I know that TFb1 and TFb2 need to dissolve individually before mixing.
by kenpier
Tue Sep 06, 2011 10:11 am
Forum: Microbiology
Topic: Making Competent Cell
Replies: 5
Views: 5404

Making Competent Cell

Hi,

Anyone here have a good protocol in making chemical treated competent cells fast?

Currently using rubidium chloride method.
by kenpier
Tue Sep 06, 2011 9:47 am
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 579469

New in Cell Biology

Hi guys, i'm new here specially in the field of Cell Culture and Microbiology. Hope i can get to learn stuff that i can apply at work. Currently working in a Mechanobiology Institute. after 2 years in pharmaceutical plant