Search found 30 matches

by greatmicrobiologist
Tue Mar 26, 2013 4:03 pm
Forum: Microbiology
Topic: incubating streptomyces lydicus
Replies: 4
Views: 4943

Re:

roscoman wrote:i was going to try to cultivate the spores from a product called actinovate. anyway, thanks for the help great microbiologist, im going to order some of that.


Check the ingredients of those media, if you already have then there is no use to buy one. Anyway have a good luck :) .
by greatmicrobiologist
Thu Mar 14, 2013 2:48 pm
Forum: Microbiology
Topic: incubating streptomyces lydicus
Replies: 4
Views: 4943

Re: incubating streptomyces lydicus

I am little bit confused of what you have mentioned. If you already have the fungi then how you have maintained it? Anyway, normally for fungal growth and maintenance you need to grow the fungi at 26-28C temparature for incubation on Potato Dextrose Agar (PDA). While you carry out with some experime...
by greatmicrobiologist
Sun Mar 10, 2013 2:30 pm
Forum: Microbiology
Topic: growing bacteria in petri dishes
Replies: 8
Views: 36019

Re: growing bacteria in petri dishes

I am absolutely supporting of what Mr Moderator has said. Working with germs require very much aseptic conditions, sometimes we Microbiologists have to wear gloves for handling cultures. As a part of your project concerned I don't know the motive of your project and from where you have isolated the ...
by greatmicrobiologist
Sat Mar 09, 2013 2:02 pm
Forum: Microbiology
Topic: How to proceed to check for its Probiotic ability
Replies: 0
Views: 2265

How to proceed to check for its Probiotic ability

I have collected two bacteria and two fungal cultures collected from sea water (Bay of Bengal) , grown on Minimal salt media. I wanted to test them whether they are Probiotic cultures or not. As Food Microbiology is very new to me. I have not yet work out with Food Microbiology. I would be glad if y...
by greatmicrobiologist
Thu Feb 02, 2012 2:11 pm
Forum: Microbiology
Topic: Fungal Contamination on jaggery
Replies: 3
Views: 3412

any suggestions to know it? or is it Aspergillus? what you think? At least genus name I want to be okay for my further experiment.
by greatmicrobiologist
Thu Feb 02, 2012 12:43 pm
Forum: Microbiology
Topic: Fungal Contamination on jaggery
Replies: 3
Views: 3412

Fungal Contamination on jaggery

I have isolated a fungal contamination from jaggery and streaked on sabouraud dextrose agar plate. It was light chocolate brown in color on the jaggery. I have stained the fungi with Lactophenol cotton blue and observed under 100X of Bright-field microscope. I wanted to know the exact species name. ...
by greatmicrobiologist
Wed Sep 21, 2011 2:02 pm
Forum: Molecular Biology
Topic: general QPCR problem
Replies: 9
Views: 10318

i feel like that the house keeping gene, Actin you are using is having some genomic sequences that is matching with your gene of interest. So my question is are you sure about the proper sequence of actin you are using?
by greatmicrobiologist
Mon Sep 12, 2011 12:39 pm
Forum: Microbiology
Topic: Limit of e.coli on dried leaves
Replies: 4
Views: 3958

Totally depends and will vary upon the dryness of the leaves. it should be of low number as you have mentioned. I don't know about the E. coli only, but any microorganism you will rarely find in dry conditions. E. coli is an enteric and is motile. hence it will require a liquid medium, hence dry pla...
by greatmicrobiologist
Mon Sep 12, 2011 12:22 pm
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 9
Views: 16379

Re: distinguishing circular and linear DNA

Disregard this, the method I was going to suggest required running on an agarose gel, and the OP mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlifica...
by greatmicrobiologist
Mon Sep 12, 2011 12:16 pm
Forum: Molecular Biology
Topic: Relationship between structure and function of glucoes
Replies: 12
Views: 24871

Go through the glycolysis. If you through the carbohydrate metabolism then you will understand the structural functions of each monosaccharide.
by greatmicrobiologist
Thu Sep 08, 2011 12:23 pm
Forum: Microbiology
Topic: Common cold
Replies: 0
Views: 2874

Common cold

A question has arose in my mind that in common cold, upper respiratory tract infection which causes sore throat, runny nose, cough and fever; so is that innate immunity that causing runny nose? I was confident that it is adaptive immunity but later i got little confused. May be the question is easy ...
by greatmicrobiologist
Thu Sep 08, 2011 12:13 pm
Forum: Cell Biology
Topic: Nucleolus
Replies: 5
Views: 9940

Re:

Adz795 wrote:
Talcen, are you sure the nucleolus assembles the ribosomal subunits? I think that is done in the cytoplasm.



nucleolus does. .