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by gradkid
Thu Aug 25, 2011 2:00 am
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 9
Views: 17632

Re: distinguishing circular and linear DNA

Run 2 restriction digests with 2 different restriction enzymes, followed by a combination digest. Let's say enzyme A cuts your DNA into 3 fragments and enzyme B cuts it into 4. You don't know if your DNA is linear with 2 restriction sites for A and 3 sites for B or circular with 4 sites for A and 5 ...