Search found 115 matches

by GreenDog
Sun Feb 01, 2009 6:41 am
Forum: Genetics
Topic: Establishing correlation between SNP and disorder
Replies: 5
Views: 3332

Re: Establishing correlation between SNP and disorder

Chi-square wouldn't do, because as I understand the calculation needed is: What are the chances one will get the disorder if he's +/+, +/- or -/-. There are no expected ratios.
by GreenDog
Thu Jan 29, 2009 1:29 pm
Forum: Genetics
Topic: Establishing correlation between SNP and disorder
Replies: 5
Views: 3332

This is a really tough question, you've asked. this is a new area and not a very established one, you should search the pubmed you'll find some articles which have done this but I find them hard to understand. a girl in a lab next to me is working on the same problem, she has 5 SNP's... Maybe you ca...
by GreenDog
Thu Jan 29, 2009 1:19 pm
Forum: Molecular Biology
Topic: bradford assay of plant protein
Replies: 1
Views: 2031

You can use water. 0.8mlDDw+0.2mlBSA+4microliter of desired protein. If you are over the scope of your standard curve or spectrometer dilute your protein 1/5 or 1/10, or whatever you like. Make standard curve with the BGG 1,2,4,8, microgram (or something similar). Follow the instructions i.e. don't ...
by GreenDog
Thu Jan 22, 2009 6:02 pm
Forum: Genetics
Topic: Proving Existence of Introns!
Replies: 4
Views: 2387

Radioactive but nice.
by GreenDog
Thu Jan 22, 2009 5:55 pm
Forum: Molecular Biology
Topic: immunoblotting and Elisa assay
Replies: 3
Views: 1681

Very, Very, Very accurate.
If you have a good antibody you'll see exactly your protein.
by GreenDog
Wed Jan 21, 2009 11:53 am
Forum: Molecular Biology
Topic: Protein degradation
Replies: 5
Views: 4971

I wouldn't leave it on the bench o.n. however you can vortex and put in 37deg for half an hour or so. It actually depends on what you'll do next and on the bugger (for western we put for 5 min in 95 and it's ok, right?).
You could also try a different precipitation method.
by GreenDog
Wed Jan 21, 2009 11:11 am
Forum: Molecular Biology
Topic: sequence of 800F, 800R
Replies: 3
Views: 1820

When I started looking I found only some of them, and sometimes with contradictions. What are they for, and for which organism are they?
by GreenDog
Tue Jan 20, 2009 2:31 pm
Forum: Molecular Biology
Topic: Protein degradation
Replies: 5
Views: 4971

Could you be more specific?
Are you purifying proteins, how? Why would you want to leave them at room temp? During the day nothing will happen to them.
by GreenDog
Tue Jan 20, 2009 9:37 am
Forum: Molecular Biology
Topic: sequence of 800F, 800R
Replies: 3
Views: 1820

Go to http://www.ncbi.nlm.nih.gov/
And search them under UniSTS.
by GreenDog
Sun Jan 18, 2009 6:55 am
Forum: Molecular Biology
Topic: Multiple genes cloning
Replies: 7
Views: 7356

I thought you just needed to get the proteins doesn't matter how and you could use two hosts. If you want all of them together on the same plasmid and in the same host it's a different question entirely. If you can choose the protein, pick an antibacterial gene which works on gram positive cells, an...
by GreenDog
Thu Jan 15, 2009 9:33 am
Forum: Molecular Biology
Topic: Multiple genes cloning
Replies: 7
Views: 7356

1. Yes it is possible. 2. As for sources, it's not written someplace people just know how to do this and they teach each other, you can also look at the sites of various biotech companies which sell stuff for molecular biology. 3. The antifungal and antiviral proteins may be transfected to bacteria,...
by GreenDog
Wed Jan 14, 2009 10:05 am
Forum: Genetics
Topic: stem cell identity.
Replies: 2
Views: 1217

You should have studied in your course something related to this question, you should have some direction. You should start thinking about a fool experiment and start describing it. I'll start: Take stem cells; put them in a medium which will cause differentiation (for example muscle cell extract to...