Search found 140 matches

by mkwaje
Sun Jan 31, 2010 5:10 pm
Forum: General Discussion
Topic: ..
Replies: 7
Views: 3264

I don't understand what you are trying to do. If you want to select for yeasts that can metabolize lactose, then do what JackBean said. Use minimal medium + lactose as the sole C source. Then you said you also wanted to grow bacteria on it? Meaning you have a mixed population at the start? For bacte...
by mkwaje
Sun Jan 31, 2010 3:59 pm
Forum: Evolution
Topic: The Mule Theory.
Replies: 8
Views: 8889

I really can't follow your logic. Since when do you base your "scientific theory" about humans using other animals as examples. If A and B produces C; it doesn't follow that D and E produces F. Because A, B,D, and E are different. That's basic logic. ... So the evolutionist scientists shou...
by mkwaje
Sun Jan 31, 2010 3:41 pm
Forum: Molecular Biology
Topic: mutation
Replies: 3
Views: 2230

If you are looking for auxotrophs, replica plating is the best. You can inoculate a lot of isolates in several diff media (like minimal medium, etc) in one go.
by mkwaje
Sat Jan 30, 2010 11:32 am
Forum: General Discussion
Topic: "Twenty questions"-biology game
Replies: 771
Views: 465863

Hi guys! If its an animal, then it won't be unicellular and it does not matter if its dead or alive. We could try to ask questions to classify it.

So my first question is

1. is it a vertebrate?
by mkwaje
Sat Jan 30, 2010 11:22 am
Forum: General Discussion
Topic: Are mitochondria in prokaryotes and eukaryotes the same?
Replies: 7
Views: 4516

I agree with the previous post that some people 'consider' the mitochondria as an ancient prokaryote engulfed by another and what resulted was a symbiotic relationship. That is how they theorized the formation of the other organelles, like chloroplasts inside eukaryotic cells. One thing that support...
by mkwaje
Sat Jan 30, 2010 11:14 am
Forum: General Discussion
Topic: ..
Replies: 7
Views: 3264

Do I understand that you are trying to make a plate with just lactose as C source? Well, you have to add other ingredients like N source unless your yeast is a N-fixer, it cannot get N out of the air, even then pure lactose and agar won't likely support your yeast... You can use minimal medium and t...
by mkwaje
Sat Jan 30, 2010 10:49 am
Forum: Microbiology
Topic: similarities between bacteria and humans
Replies: 7
Views: 12370

Re:

and there's always population dynamics if the molecular similarities are too straightforward. I don't think population dynamics of bacteria and human are the same. We can't replicate on our own like bacteria and bacterial interactions are much much simpler than in humans. To answer the question, ma...
by mkwaje
Sat Jan 30, 2010 10:41 am
Forum: Microbiology
Topic: dialogue for short film
Replies: 4
Views: 5705

Re: dialogue for short film

hmm. this dialogue is really far out.. my small comments, though I'm not a virologist. at the last part, its not the virus that are competent, those are for the cells that will be infected. If you have competent cells, then those cells can be transformed (undergo transformation) whereby the naked vi...
by mkwaje
Sat Jan 30, 2010 10:25 am
Forum: Microbiology
Topic: Cell number from dilution factor.
Replies: 4
Views: 4463

Re: Cell number from dilution factor.

Actually using the old method of bacterial enumeration. you have to consider only the plates containing colonies between 30-300.. For fungal plates, they adjusted it to 25-250... At these colony numbers, there is a higher chance of getting the correct CFU. Discard the plates with lower than 30 or hi...
by mkwaje
Fri Jan 29, 2010 10:20 pm
Forum: Microbiology
Topic: antibiotic research
Replies: 9
Views: 6011

I once read that you can observe evolution when you stare at it long enough. A group of scientists found out that after 31,500 generations, evolution took place.Similarly, I find it intriguing if we force a bacteria to adapt to form an antibiotic against human pathogens. The most likely step is to s...
by mkwaje
Fri Jan 29, 2010 9:55 pm
Forum: Microbiology
Topic: Which conditions promote QS in Pseudomonas aeruginosa?
Replies: 1
Views: 1505

Hi...we studied quorum sensing once. It was interesting but we didn't get to experiment on it. Here is an article about QS in Pseudomonas. Maybe you can find something useful in there. m In some cases like S. aureus, you have to reach a certain density (like 10^8 cells) for it to produce toxin. I ju...
by mkwaje
Fri Jan 29, 2010 9:48 pm
Forum: Microbiology
Topic: plantae protist and diatoms evolution
Replies: 3
Views: 15750

What is your question? You asked the question and then attempted to answer it using Freeman's Biological Science. I assume this is a question posed by your teacher and you are looking for opinions that you are on the right track. Well, you can't argue with a book, and you would better discuss your p...