Search found 4 matches

by kitkat11
Wed Mar 23, 2011 11:51 pm
Forum: Molecular Biology
Topic: final concentration
Replies: 16
Views: 10935

Re: final concentration

ok so i think i understand ... im just want to check. so if i do the 0.6 ml of 1% lactose and put that in 40 ml of ecoli culture.... then: i take 0.001 / 132 = 7.57 x 10^-6 and then i times that by 0.006 = 4.5 x 10^-8 and divide that by 0.0406 = 1.1 x 10^-6 which would be 1.1 uM? sorry ... im just h...
by kitkat11
Wed Mar 23, 2011 9:36 pm
Forum: Molecular Biology
Topic: final concentration
Replies: 16
Views: 10935

Re: final concentration

ok so i think i get that formula ... but how does moles then relate to percentage. so like for lactose it has a molecular weight of 132 (roughly) and the only formulas im thinking of include n=m/M and c=n/v .... but i don't know how that relates to percentages?
by kitkat11
Wed Mar 23, 2011 1:57 pm
Forum: Molecular Biology
Topic: final concentration
Replies: 16
Views: 10935

Re: final concentration

yes i noticed that mistake but i was so confused let people see where i was going wrong ... umm can u explain that formula u gave though in a bit more detail (or e.g.) because i've never seen it? or heard of it? thanks!
by kitkat11
Wed Mar 23, 2011 12:51 pm
Forum: Molecular Biology
Topic: final concentration
Replies: 16
Views: 10935

final concentration

I'm confused with working out the final concentrations of these. I added these to 6 separate flasks each containing 40 ml of ecoli in a glycerol medium: a. 2.0 ml (10 mM) IPTG b. 0.6 ml (1%) lactose c. 0.6 ml (1%) lactose and 0.5 ml (8%) glucose d. 0.6 ml (1%) lactose and 1.0 ml of 5-FU e. 0.6 ml (1...