Search found 3 matches

by maheshgen
Tue Mar 15, 2011 10:51 am
Forum: Molecular Biology
Topic: Molecular biology
Replies: 6
Views: 2956

Re:

seems rather like problem with the vector. How did you check you have really the vector you want? How was your DNA purification? Are you sure the vector is as from manufacturer, wasn't it cut already in the past? we use these vectors and they all function well. Even i cut these before without any d...
by maheshgen
Mon Mar 14, 2011 9:22 pm
Forum: Molecular Biology
Topic: Molecular biology
Replies: 6
Views: 2956

Re:

they seem fine, both Fermentas and NEB Enzymes show they should work fine in wide range of buffers, so that shouldn't be a problem. What about other conditions? How do you check the result? Where did you get the plasmid? Thanks jackBean for reply. The enzymes are working. We have pET-28 a-c (+) vec...
by maheshgen
Mon Mar 14, 2011 1:48 pm
Forum: Molecular Biology
Topic: Molecular biology
Replies: 6
Views: 2956

Molecular biology

I have a problem in restricting pET-28a(+) and pET-28b(+) vectors with NcoI and XhoI. I have tried Fermentas enzymes with buffer Tango. I tried many times but went invein. Can anybody guide me in this as I am in desperate need of this vector to proceed my work. Thank you in advance for the advice