Search found 20 matches

by seaseasea
Sun May 13, 2007 2:22 pm
Forum: Molecular Biology
Topic: mutation of psb
Replies: 6
Views: 4197

OK. I think your question is very professional and many viewers may be unfamiliar with this. I think you could search reference database to find what you need. Hope this helps.
by seaseasea
Sun May 13, 2007 2:17 pm
Forum: Molecular Biology
Topic: cloning troubles
Replies: 5
Views: 2464

In my opinion, I think it's may be difficult to digest PCR products with REase when no extra bases existing in the terminal of primers. I donlt know whether no growth on plates were caused by this.
Moreover, I advice you clone the PCR products directly into cloning vectors to make a sequence.
by seaseasea
Thu Jul 13, 2006 9:43 am
Forum: Molecular Biology
Topic: Need Help? Plasmid from Gram(+)
Replies: 5
Views: 2552

Yes,addition of lysozyme is very easy.Generally,I put lysozyme in solution I.This method is used in our lab to isolation plasmid from Bacillus subtilis.
by seaseasea
Wed Jul 12, 2006 7:42 am
Forum: Microbiology
Topic: Amino acid synthesis in bacteria
Replies: 9
Views: 17280

E.coli could synthesize 20 amino acids required for synthesis of proteins.If the environment is rich in one or two or all of 20 amino acids,E.coli doesn't sythesize those amino acids. E.coli could use carbon resources and nitrogen resources to make amino acids through metabolic pathways.Those reacti...
by seaseasea
Wed Jul 12, 2006 7:32 am
Forum: Microbiology
Topic: What Influences transcription rate in bacteria?
Replies: 2
Views: 3067

Hello neon,
This is the second time today I meet you.
I still want to say lots of factors influencing transcription rate in bacteria.
You could look up monograph or other references.
Hope this helps!
by seaseasea
Wed Jul 12, 2006 7:23 am
Forum: Molecular Biology
Topic: Chemicals influencing transcription rate
Replies: 1
Views: 2011

Hello neon, After looking message above,I feel the works you will do would be complicated.First of all,there are lots of factors affecting transcription rate and more factors affecting protein production.Secondly,every protein is sepcial.Factors affecting one protein production significantly is diff...
by seaseasea
Wed Jul 12, 2006 6:43 am
Forum: Molecular Biology
Topic: P16, hMLH, and E-Caderin, What are the specific patrhways
Replies: 1
Views: 1079

Your question is hard to answer because of lots of friends not experts in this area.I guess you could search PubMed through keywords such as P16,hMLH and E-Caderin or look up monograph about the area.Hope this helps!
by seaseasea
Wed Jul 12, 2006 6:34 am
Forum: Molecular Biology
Topic: How do primers work?
Replies: 5
Views: 26916

PCR is a powerful technique and is widely used in molecular biology.Looking a monograph about PCR would be helpful to you.Hope this helps!
by seaseasea
Wed Jul 12, 2006 6:28 am
Forum: Molecular Biology
Topic: kanamycin resistance problems.
Replies: 7
Views: 3679

hello,
freezed kana could be no problem.i usually use freezed kan.
phenomenon like this is really rare.
by seaseasea
Sun Jul 09, 2006 6:05 am
Forum: Molecular Biology
Topic: Mechanisms of protein secretion
Replies: 0
Views: 1227

Mechanisms of protein secretion

I'm working on mechanisms of protein secretion and engineering of protein secretion pathways.A friend in this field is indispensable.Who are you?My friend,I'm waiting you here.[/i][/b]
by seaseasea
Sun Jul 09, 2006 5:43 am
Forum: Molecular Biology
Topic: Europe's Opposition to Crop Biotechnology
Replies: 5
Views: 2308

Anything which is helpful to human should be developed all over the world.Biotechnology is also a powerful tool to improve the life of human beings.
by seaseasea
Tue May 16, 2006 5:57 am
Forum: Microbiology
Topic: How do viruses cause diseases
Replies: 5
Views: 12461

i think there are many mechanisms used by viruses to result in deaeases.it also can secretes peptide which has a role in cell signl transduction
pathway.