Search found 90 matches

by oppox
Mon Feb 05, 2007 8:23 am
Forum: Molecular Biology
Topic: loss of Ecoli over-expression
Replies: 2
Views: 1404

to inhibit expression u could try tuner or rosetta cells (these are from novagen other companies have different ones) they contain an extra plasmid for expression of the pet-blue which lacks the T7 thingy. Though rosetta is also a rare codon cell strain so maybe tuner is the first to start with.
by oppox
Sun Jan 14, 2007 2:56 am
Forum: Molecular Biology
Topic: 5' to 3' or 3' to 5' or wtf?
Replies: 7
Views: 2442

yes its read 3' to 5' but u can say the replication is done 5' to 3' if u refer (spelling) to the new strand (its growing 5' to 3')
check this up http://en.wikipedia.org/wiki/Dna
hope it helps and im hoping im not wrong about it :)
by oppox
Sat Jan 13, 2007 5:53 pm
Forum: Molecular Biology
Topic: Need advice in primer designing
Replies: 3
Views: 1758

its hard to know where to start without seeing the gene. the forward primer should be located in the beginning and the reverse in te end of the gene. thats pretty much it. Its hard to say anything but that right know since I dont know what u are going to do with it, are u having primers with a restr...
by oppox
Thu Jan 04, 2007 10:06 am
Forum: Molecular Biology
Topic: Expressing protein !! Help!
Replies: 2
Views: 1488

one thing that may be worth looking at is the codon usage, see if u have many rare codons.
by oppox
Sun Dec 17, 2006 2:25 pm
Forum: Molecular Biology
Topic: enzyme denatureization
Replies: 9
Views: 3275

I my self thought it to be safe to store proteins in a freezer, but then my friend told me about his protein. Also there are something called cold denaturation.
by oppox
Fri Dec 15, 2006 2:53 pm
Forum: Molecular Biology
Topic: minimum possible size of a plasmid
Replies: 2
Views: 1859

there are plasmids about 1 kb, but I dont know if its a minimum for it to circularize
by oppox
Wed Dec 13, 2006 1:11 am
Forum: Molecular Biology
Topic: His-tag on primer?
Replies: 4
Views: 7180

you might have too incorporate a trombin site to if u want to be able to cut the his tag away. I have thought of this too when I had problems, why do u want the his-tag on your primers, is it just an idea?
by oppox
Mon Dec 11, 2006 9:31 pm
Forum: Molecular Biology
Topic: insoluble proteins
Replies: 4
Views: 8711

inclusion bodies are insoluble proteins aggregates (not soluble in "water"), the protein forms aggregates when expressed (some proteins have a hydrofobic core but when they arent correctly folded, the core is exposed and can bond through non hydrophobic bonds with other non folded proteins...
by oppox
Sun Nov 26, 2006 11:59 pm
Forum: Molecular Biology
Topic: enzyme denatureization
Replies: 9
Views: 3275

I know that a friend of mine happened to denaturate his protein when he tried to store it in a freezer, however it dont apply to all proteins. Taq polymerase for instanse is stored below zero, it comes from a heat resistent bacteria. Its hard to say if yours will denaturate.
by oppox
Fri Nov 17, 2006 3:49 pm
Forum: Molecular Biology
Topic: pJIT60 nucleotide sequence
Replies: 2
Views: 1921

here, save it and open it in words, sunno if there is a better program.
http://www.pgreen.ac.uk/JIT/35S-plasmid.htm
by oppox
Thu Oct 26, 2006 11:54 pm
Forum: Molecular Biology
Topic: Drawing structures
Replies: 4
Views: 1641

yes I cant see any wrong with your answer except the S.

I havent found it written as u got in this assignment, CH2CH2CO...
only as structural forms so I cant answer that question for sure.
by oppox
Thu Oct 26, 2006 9:52 pm
Forum: Molecular Biology
Topic: Drawing structures
Replies: 4
Views: 1641

well eh no... I dont have this completly fresh but, havent u forgotten the 16 carbons? you said u were given CH3(CH2)11CH2...... was that all, that doesnt do much except making me not understanding why its given. oh now I understand, u were going to draw the rest of the molecule....bing bing the coi...