Search found 22 matches
- Thu Mar 08, 2007 8:08 pm
- Forum: Cell Biology
- Topic: Cell motility/invasiveness assay?
- Replies: 2
- Views: 2589
I've heard of matrigel, although I guess I would have to order a kit for that? isn't it expensive? Aren't micro-boyden chambers used to study chemokine effects on leukocytes? I just want to test the effects of chemotherapy drugs on the cell motility and invasiveness...can boyden chambers be used for...
- Wed Mar 07, 2007 6:37 pm
- Forum: Cell Biology
- Topic: Cell motility/invasiveness assay?
- Replies: 2
- Views: 2589
Cell motility/invasiveness assay?
Hello experts, I am doing some tissue culture work with cancer cells and was wondering if anyone knew of any quick and simple methods to measure cell motility or invasiveness? A lot of the assays for cell motility seem too time consuming. As a last resort, I suppose there are kits out there that ser...
- Tue Oct 03, 2006 6:36 pm
- Forum: Molecular Biology
- Topic: incomplete transfer of protein samples for western blot
- Replies: 0
- Views: 1576
incomplete transfer of protein samples for western blot
Hi all, I've been having a great deal of problem transferring my proteins over to my nitrocellulose membrane for western blots. The strange thing here is that my ladder has actually completely transferred over and even passed through the membrane while my protein samples are all mostly stuck onto th...
- Thu Jul 13, 2006 5:29 pm
- Forum: Molecular Biology
- Topic: Melting Curve giving fluctuating Ct values
- Replies: 1
- Views: 1485
Melting Curve giving fluctuating Ct values
Hi all, I am doing some QPCR experiments with SYBR Green using cDNA and my primers never seem to work properly. I re-designed it 3 times already, the first time the primers looked promising- from the melting curve there were no primer dimers but then the CT values were flutuating up and down for my ...
- Mon Jun 12, 2006 6:11 pm
- Forum: Molecular Biology
- Topic: Is it ok to replate from a bacterial lawn?
- Replies: 2
- Views: 2164
- Fri Jun 09, 2006 3:01 pm
- Forum: Molecular Biology
- Topic: Is it ok to replate from a bacterial lawn?
- Replies: 2
- Views: 2164
Is it ok to replate from a bacterial lawn?
Hi all, I was doing some plating on LB plates and let it grow overnight. The next day I noticed that I had a lawn of bacterial growth, probably becuase I plated the entire transformation mix and it was way too concentrated. Anyhow, I was just wondering whether it would be ok to replate from this bac...
- Thu Jun 08, 2006 5:05 pm
- Forum: Molecular Biology
- Topic: Vector is 80X larger than insert, what molar ratio to use?
- Replies: 9
- Views: 9238
I am transforming into DH5alpha competent cells, I think the ligation mix is alright...I'm trying it with fresh buffer and ligase in a 20ul ligation mix at 22 degrees for an hour now at various molar ratios. I re-made a fresh batch of insert and vector both around 30ng/ul. If I used 1ul of both inse...
- Mon Jun 05, 2006 3:29 pm
- Forum: Molecular Biology
- Topic: Vector is 80X larger than insert, what molar ratio to use?
- Replies: 9
- Views: 9238
- Mon Jun 05, 2006 2:57 pm
- Forum: Molecular Biology
- Topic: smearing in bands after digestion
- Replies: 3
- Views: 2429
- Fri Jun 02, 2006 9:00 pm
- Forum: Molecular Biology
- Topic: smearing in bands after digestion
- Replies: 3
- Views: 2429
smearing in bands after digestion
Hi everyone, I was wondering why I keep seeing a smear in my bands after digestion of my vector. I digested with 1ul EcoR1 and XhoI in a 50ul reaction for 2-3 hours. I also ran the uncut vector along side it and noticed that the smear appears only after digestion. My post-doc told me to just cut the...
- Fri Jun 02, 2006 4:42 pm
- Forum: Molecular Biology
- Topic: Vector is 80X larger than insert, what molar ratio to use?
- Replies: 9
- Views: 9238
- Wed May 31, 2006 9:21 pm
- Forum: Molecular Biology
- Topic: Vector is 80X larger than insert, what molar ratio to use?
- Replies: 9
- Views: 9238