Search found 13 matches

by Awol
Thu Nov 22, 2012 3:58 pm
Forum: Molecular Biology
Topic: Could someone suggest a therapeutic protein expressed by bio
Replies: 1
Views: 1775

Could someone suggest a therapeutic protein expressed by bio

As part of my Molecular Biology course I have been looking for some examples of therapeutic proteins that are now produced industrially as a result of biotechnology, Insulin and HGH are the obvious ones, but I would like to do a bit of further reading on some less obvious ones. Nothing too advanced ...
by Awol
Mon Sep 17, 2012 10:41 am
Forum: Molecular Biology
Topic: First time making a genomic library
Replies: 2
Views: 2928

First time making a genomic library

I am a molecular biology student and I have been making genomic libraries in E.coli for the first time. I was posed a question by my prof. that has confused me. I am asked whether this approach, using two different restriction enzymes, could be used to generate a complete genomic library? And if not...
by Awol
Wed Apr 18, 2012 6:18 pm
Forum: Microbiology
Topic: What's the chemical reaction catalysed by nitrate reductase?
Replies: 1
Views: 5782

What's the chemical reaction catalysed by nitrate reductase?

subject says it all really. i have been reading about the difference in energy released as per this chart http://i.imgur.com/ug3uF.png . I assume that in aerobic organisms the chemical reaction would be
(NADH + H + 1/2O2) -> (NAD+ + H2O) but how does this work with nitrite reductase in anaerobic respiration?
by Awol
Wed Apr 11, 2012 3:34 pm
Forum: Molecular Biology
Topic: What in your opinion is the most exciting new biotechnology?
Replies: 0
Views: 1617

What in your opinion is the most exciting new biotechnology?

I am interested to hear what people have to say about new biotechnology and its applications. I personally would love to hear about new recombinant DNA technology that escapes the headlines but in your opinion has great implications for the future.

I look forward to hearing what you have to say.
by Awol
Wed Apr 11, 2012 3:28 pm
Forum: Molecular Biology
Topic: Wobble hypothesis.How does met & trp not get mis translated?
Replies: 2
Views: 9709

This makes a lot more sense to me now. My reasoning was that there was 61 different aminoacyl-tRNAs in the cell i see know how i why i was getting confused. Thank you for taking the time to explain this to me.
by Awol
Sun Apr 08, 2012 6:33 pm
Forum: Molecular Biology
Topic: Wobble hypothesis.How does met & trp not get mis translated?
Replies: 2
Views: 9709

Wobble hypothesis.How does met & trp not get mis translated?

I understand the principle of the wobble hypothesis, that the last base pair between a mRNA codon and a tRNA anticodon is not as stringent as the DNA base pairing rules. However the rules as they were explained to me do not compensate for met & trp. If the tryptophan codon = (5'-UGG-3') then the...
by Awol
Fri Mar 30, 2012 3:58 pm
Forum: Molecular Biology
Topic: I need some actual DNA sequences for a computer program.
Replies: 7
Views: 5904

If not sure if I follow what you are trying to say....

If the random sequence is AUGAAAGGUAG (with AUG = Start and UAG = Stop) the 'gene' = AAAGG. Which is
5 bases = 2/3 codons. Where as an actually DNA sequence will tend to have genes of multiples of 3 unless mutated.
by Awol
Fri Mar 30, 2012 1:10 pm
Forum: Molecular Biology
Topic: I need some actual DNA sequences for a computer program.
Replies: 7
Views: 5904

I have been using random sequences up until now. Its easy enough to pick out a gene looking for start/stop codons (or Shine-Dalgarno sequences) but the 'gene' it identifies is not a multiple of 3 bases. The program then assumes a deletion/insertion mutation (or equivalent) resulting an erroneous ami...
by Awol
Thu Mar 29, 2012 9:14 pm
Forum: Molecular Biology
Topic: I need some actual DNA sequences for a computer program.
Replies: 7
Views: 5904

I need some actual DNA sequences for a computer program.

I am currently writing a computer program to search DNA sequences. i need some DNA to test my code on. Any ideas where I could get a few thousand pb from?
by Awol
Tue May 31, 2011 2:59 pm
Forum: Cell Biology
Topic: Book on iPS (induced pluripotent stem cells) 2ndYr BioStu
Replies: 1
Views: 1767

Book on iPS (induced pluripotent stem cells) 2ndYr BioStu

i would like to read about iPS in more detail. All the books i have found so far are either to basic or too advanced for my level of knowledge. I am a 2nd year biology student. I would really like some recommendations
by Awol
Fri Dec 10, 2010 12:45 pm
Forum: Microbiology
Topic: Arsenic-eating microbe
Replies: 12
Views: 9350

Re: Arsenic-eating microbe

Here on the Earth water seems to be pretty damn crucial. At least I cannot think of any example a life form that would be able to carry on its metabolic functions in the complete absence of water. But then again, we don't really have liquid ammonia or methane lakes here for life to have a try. Mayb...
by Awol
Fri Dec 03, 2010 6:07 pm
Forum: Microbiology
Topic: Arsenic-eating microbe
Replies: 12
Views: 9350

Re: Arsenic-eating microbe

So it just uses Arsenate ( AsO4^3− ) instead of Phosphate ( PhO4^3- ) ? This is fascinating stuff, however I cant help but feel we shouldn't be surprised that life had adapted to flourish in more inhospitable environments, it has a good track record when it comes to that sort of thing What do you th...