Search found 5 matches

by andresgutierrez
Thu Dec 02, 2010 7:28 pm
Forum: Molecular Biology
Topic: IC50
Replies: 2
Views: 2173

Hello,

You should plot a graph - X axis - concentration of drug and Y- axis - cell viability - ( Abs ( with drug) / Abs(control without drug addition ) *100%

So you can estimate Ic 50 as a concentration of drug at 50% position on Y axis.

Andres
by andresgutierrez
Thu Dec 02, 2010 7:24 pm
Forum: Molecular Biology
Topic: designning primers
Replies: 5
Views: 3065

Perhaps this video may help you but the primers design requiere a lot of attention. m Some advices, 1. Select a conserved regions from your alignment. 2. A good primers sequences has: 18 - 22 nt, 55°C as melting temperature, without second structures but If have it be careful that they occurs under ...
by andresgutierrez
Thu Dec 02, 2010 7:15 pm
Forum: Bioinformatics
Topic: KEGG metabolic pathway database
Replies: 2
Views: 3135

Read about KEGG first at wiki this is the link: http://en.wikipedia.org/wiki/KEGG

Then you can visit the website

http://www.genome.jp/kegg/

regards,

Andres
by andresgutierrez
Thu Dec 02, 2010 7:11 pm
Forum: Bioinformatics
Topic: know about bioperl
Replies: 7
Views: 5586

Re: know about bioperl

Bioperl is a package that you can download from http://www.bioperl.org Do you already know how to program using perl? To use Bioperl you have to call the program from your shell open the libraries select the tool you need and run it.

For example use Bio::Seq

regards,

Andres
by andresgutierrez
Thu Dec 02, 2010 7:04 pm
Forum: Bioinformatics
Topic: Worth it to learn some python for a biochemist?
Replies: 2
Views: 3256

Re: Worth it to learn some python for a biochemist?

You have to focus first on your biological question. So many times I talked with students around the world struggling with the learning of a programing lenguaje without any idea about data structures and algorithms design, So ask yourself Why do you want to learn to program?. As an advice, I recomme...