Thanks for the response, canalon. Cytochrome C degradation makes sense seeing as the smear started where the band should have been, and continued past it. Could you explain to me how degradation might happen?

I ran an SDS-PAGE for my lab, and in two of the wells I loaded a Cytochrome C/buffer mixture. Both of these lanes resulted in a light smear starting at approx halfway down the gel and ending approx 3/4 of the way down the gel. What would be the reason for the smear? Note: All of my other protein ban...

Yes, exactly. We have learned about the variations to Mendel's principles, but they aren't being applied in this first assignment. Only the basic "Mendelian" should be used in this case. What I thought was, since my prof said that the 7 F1 offspring (4 black:3 pink) was a 1:1 ratio (crossi...

There isn't any more information, but I'm pretty sure this assignment doesn't cover the variations to Mendel's Principles. Just the basic, "pea plant" scenarios. (I'd thought of lethal genes too.)

In rats, a black-tailed mutant was discovered. When it was crossed to a pink tailed rat, 4 offspring were black-tailed and 3 were pink tailed. Two of these black-tailed rats were crossed and they produce 6 black tailed and 3 pink-tailed rats. These experiments were repeated several times with approx...

Hi there, Why might my uncut lambda DNA have gotten stuck in my agarose gel well? When I had the gel visualized and a picture printed of my fluorescing bands, I noticed that my uncut lambda DNA had not moved from the well, except for a light band that did not even travel as far as the band depicting...

The full question is this: "You are a summer research student in a molecular biology research lab. You are asked to insert the "stresslessontests" gene into a plasmid. It is important that the gene is inserted in the proper orientation so that a functional protein can eventually be ma...

Scenario: You digest DNA into fragments and cut plasmids at a specific site, then mix them together and add ligase to hopefully insert a DNA fragment into a plasmid and close it up. How do you ensure that the DNA is inserted in the proper orientation (not backwards?) Assume the DNA has sticky ends a...

That makes sense, but we were specifically told that we would not be using glass pipettes (just estimating the amount of CaCl2 according to the lines on our centrifuge tube) and that vortexing would kill our cells. So I still don't know what to do.

For my lab tomorrow there's a step that says: "Add ~2mL of ice cold 0.1M CaCl2 to the cell pellet and gently resuspend the cells." I know what resuspending means but I don't know how to go about doing this, as we are supposed to pour the CaCl2 straight into the tube (approx ~2mL) and can't...

Hi there, I've memorized the names and the one letter codes for all 20 amino acids, but would someone be able to tell me the properties (hydrophobic, polar, or ~neutral) of all the side chains? Every web site I go to says something different, and my professor didn't add that to his slides. I know th...

That makes sense.
But continuing on from that point: Does each colony of bacteria differ from one another? Could that be another way that the culture would become "contaminated" by using 2 or 3 colonies? Or are the colonies pretty much identical?
Thanks.